Antibodies and Reagents
Pre-conjugated antibodies are available from Fluidigm and other companies. Fluidigm also provides pre-designed panel kits, sample prep reagents, barcoding kits and Max-Par antibody conjugation kits to conjugate your own antibodies.
Check the links below for reagents from several companies.
You can also contact us for consultation suited to your experimental needs!
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Protocols
Protocols for cell staining for surface, nuclear signaling proteins and phosphoproteins can be found on Fluidigm website by scrolling down to the CyTOF section. The Nolan Lab at Stanford has also many protocols related protocols availabl. You contact the BRFAA CyTOF lab for locating the protocol that suits you need.
You can also contact us for consultation suited to your experimental needs!
Data analysis Tools
Data analysis can be performed using commercially available software such as Cytobank (cloud-based online platform), FlowJo and pathsetter among others. BRFAA CyTOF lab can assist to perform exploratory and statistical inference analysis.
You can also contact us for consultation suited to your experimental needs!
CyTOF Forum
One of the best places to connect with the global CyTOF community. This forum covers a variety of topics like experimental design, instrument operation and data analysis.
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